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Schematic procedure for the immobilization of DAAO Silanized silica(10g) Adding of 25% Glutaraldehyde(30ml)/ 200ml of 0.1M potassium phosphate buffer (pH7.5) Agitating(20C *1hr) DW washing Suspending in 300ml of 0.1M potassium phosphate buffer (pH7.5) Adding of DAAO solution (200U/ml*5ml) Agitating(20C *1hr) Immobilized yield by HPLC Hanyang Univ. Immobilization of Enzyme - Schematic procedure for the silanization of silica Silica(10g) Washing(with methanol, H2O2, HNO3) Adding aminosilanes(50ml) Agitating(110C *4hrs) Washing with DW Drying(105C *2hrs) Hanyang Univ. , Time control Centrifuge (Treated gel + Water) Washing (DI water) Vacuum drying Grinding Classification Characterization Hanyang Univ.

Hydrothermal process Reactor(High Pressure) -Temp.

Illustration of pore structure before (a) and after (b) hydrothermal treatment by means of autoclave process Hanyang Univ.